The enzyme solution was recirculated with a peristaltic pump through. Hollow fibre membrane arrays for co2 delivery in microalgae. Flat sheet or hollow fibre comparison of fullscale. Hollow fiber reactors reduce cost, scaleup concerns. Us4440853a microbiological methods using hollow fiber. Frontiers nitrogen production by efficiently removing. Jul 27, 2014 hollow fibre membrane bioreactors hfb provide a novel approach towards tissue engineering applications in the field of regenerative medicine.
Design and operating criteria for hollow fiber bioreactors. The development of tissue engineering hollow fiber bioreactors hfb requires the optimal design of the geometry and operation parameters of the system. These reactors offer a very gentle means for immobilizing biocatalysts such as enzymes, animal ceils, plant cells, or microbial cells. Design, construction, modelling and control of a dualhollow.
Hollow fiber reactors allow scaleup without a proportional increase in production costs while providing economy of scale. Hollow fiber bioreactors for in vivolike mammalian tissue. Design, construction, modelling and control of a dual hollow fibre bioreactor for iybridoma cells john w. The pores of the sponge structure serve as a housing for microorganisms or cells with high. Methods for microbiological processing of organic materials for production of valuable products. Unlike hollow fibers with fingerlike straight pores used in other works wang et al.
Unlike conventional cell culture methods, the cost for hollow fiber production of one milligram decreases as production increases. Flat sheet or hollow fibre comparison of fullscale membrane bio reactor configurations. Vicaria department of chemical engineering, faculty of sciences, university of granada, 18071 granada, spain and it is possible perform a simple scaling using several units in parallel form, obtaining high productivities. Operating diagrams are presented that describe reactor performance as a function of important operating variables like oxygen concentration and the fiber packing. Hollow fiber bioreactors hollow fiber membrane modules have been used as bioreactors to pro duce or transform chemicals.
Ethanol production saccharomyces cerevisiae immobilized. Hollow fibre membrane bioreactors for tissue engineering. Abstract a highly permeable composite hollow fibre membrane developed for air separation was used in a membrane aerated biofilm reactor mabr. The in vivo hollow fiber model is a fast and economic in vivo screening approach which can be used for two different purposes. A scalable flow reactor is demonstrated for enantioselective and regioselective rhodium carbene reactions cyclopropanation and c. Hollow fiber bioreactors are the focus of scientific research aiming to mimic physiological vascular networks and engineer organs and tissues in.
Development of an online microbore hollow fiber enzyme. Hollow fibre module an overview sciencedirect topics. Effects of temperature, biotechnology and bioengineering, 39, 11, 10971111, 2004. A novel hollow fiber membrane reactor for high purity h 2 generation from thermal catalytic nh 3 decomposition miao yu pi, rensselaer polytechnic institute shiguang li co. A novel hollow fibre membrane biofilm reactor for autohydrogenotrophic denitrification of drinking water. Thereactor shells ofc20andc40 were inoculated simultaneously with an exponentialphase yeast inoculum107 cells perml. Schematic drawing of hollow fiber enzyme reactor from re.
Abstract mammalian cell culture in hollow fiber reactors is hampered by oxygen limitation. Biosynthesis of drug metabolites using microbes in hollow. Shuler, the measurement of effective substrate diffusities within whole cell suspensions using a diffusion. Pdf catalytic hollow fibre based reactors for a enhance h2. Advances in fiber construction and methods for oxygenation have the potential to bring the advantages of hollow fiber cell culture to biomanufacturing. Hollow fiber membrane life in membrane bioreactors mbr. Interfacial microfluidic processing of metalorganic. One method that can improve gas exchange within photobioreactors without excessive mixing is the use of hollow fibre membranes, which enable simultaneous transport of gases deep into the reactor and rapid exchange with the culture media. Experimental and theoretical studies on a backflush hollow. Work on enzymes, animal cells and plant cells will be men tioned only where the findings are relevant to microbial cell culture. American type culture collection 53771 in a shakeflask reactor hydroxylated diclofenac 50. Pdf hollow fiber bioreactors are the focus of scientific research aiming to mimic physiological vascular networks and engineer organs and tissues in. The hollow fibers were sealed on one end and were pressurized with hydrogen on the other end.
Production of exosomes in a hollow fiber bioreactor john j s cadwell, president and ceo, fibercell systems inc introduction exosomes are small lipid membrane vesicles 80120 nm of endocytic origin generated by fusion of cytoplasmic endosomal multivesicular bodies with the cell surface. Pdf a laboratory scale hollow fibre membrane bioreactor which used bubbleless membrane aeration was tested over 5 months for its ability. Flat sheet or hollow fibre comparison of fullscale membrane bioreactor configurations. Hollow fibre modules typically contain 50 to 5000 selfsupporting, narrowbore membrane tubes, as shown schematically in figure 11. Declaration of originality i hereby declare that the work reported in this thesis was composed and originated entirely by me. Feed enters at one end of the module and retentate leaves at. Ideal for ultrafiltration, diafiltration and microfiltration, singleuse spectrum hollow fiber filters for tangential flow filtration are packed in housings engineered to handle high pressures, with a choice of fiber chemistries and sizes from process development to manufacturing. Hollowfiber membrane an overview sciencedirect topics. Soybean milk serves as a base for a variety of beverages designed for consumption in developing countries. Syngas mass transfer analysis in a hollow fiber reactor. This hollow fiber membrane without fingerlike pores could bring higher mechanical strength. This article provides a strategy for specifying operating conditions for the system based on mathematical models of oxygen delivery to the cell population. An alternative approach is the use of hollow fiber cartridge hfc reactors.
The composite membrane consisted of a porous support layer covered with a thin dense film, which was responsible for oxygen enrichment of the permeate stream. Soybean flour contains raffinose and stachyose considered to be responsible for flatulence often associated with these products j. Its a timescalable production process thats ideal for generating significant amounts of very concentrated evsexosomes, mabs or recombinant proteins. Hollow fibre membrane arrays for co 2 delivery in microalgae photobioreactors. This thesis focuses on the development of a catalytic hollow fibre membrane reactor for the combined steam methane reforming smr and watergas shift wgs reaction, using a nibased catalyst, and at a temperature window suitable for harvesting pure h2, a clean energy carrier, from the reaction by a pd membrane. Li department of chemical engineering, imperial college london, london sw7 2az, united.
Asymmetric al 2 o 3 hollow fibres produced by a phaseinversion and sintering technique were employed as a single substrate for both coating of the pd membrane and impregnation of the 30%cuoceo 2 catalyst. H functionalization by developing cascade reaction methods employing a microfluidic flow reactor system containing immobilized dirhodium catalysts in conjunction with the flow synthesis of diazo compounds. The shellandtube structure makes heat and mass transfer. Vibration of hollow fibre membranes in submerged membrane bio reactors smbrs li tian school of civil and environmental engineering a thesis submitted to the nanyang technological university. Hollow fibre membrane bioreactors hfb provide a novel approach towards tissue engineering applications in the field of regenerative medicine. Hollow fiber bioreactor technology for tissue engineering. Theinoculum was not drained fromthe reactor shell, and both shell ports ofeach reactor were closed for the duration of the experiment so that nutrients and.
Pdf flat sheet or hollow fibre comparison of fullscale. A novel hollow fiber membrane reactor for high purity h 2 generation from thermal catalytic nh 3 decomposition miao yu pi, rensselaer polytechnic institute shiguang li copi, gas technology institute. Mammalian cell culture in hollow fiber reactors is hampered by oxygen limitation. However, implementing smallscale hollow fiber bioreactors for culturing mammalian cells can save a lot of time and money, and is especially useful for harvesting large quantities of secreted protein. Catalytic hollow fibre membrane reactors for h2 production core. Mar 15, 2007 hollow fiber reactors have been developed for many biochemical and biomedical applications. Polymeric hollow fiber membranes for bioartificial organs and tissue. Diffusive oxygen transport from the fiber wall to the cells can. Diffusive oxygen transport from the fiber wall to the. Inorganic hollow fibre membranes in catalytic processing. Asymmetric hollow fibers are employed in a flow reactor, where the hollow fibers have a semipermeable membrane surrounding a lumen, where the semipermeable membrane is supported by a sponge structure. Fluorimetric determination of amino acids by highperformance liquid chromatography using a hollowfibre membrane reactor.
For adherent cell types, hfbs offer an in vivolike microenvironment as each fibre replicates a blood capillary and the mass transfer rate across the wall is independent from the shear stresses experienced by the cell. A fibercell systems hollow fibre bioreactor enables the continuous culture of large numbers of cells at high density. Hollow fiber membrane modules have been used as bioreactors to pro duce or transform chemicals. A hollow fiber bioreactor is a 3 dimensional cell culturing system based on hollow fibers, which are small, semipermeable capillary membranes arranged in parallel array with a typical molecular weight cutoff mwco range of 1030 kda. A side view of a series of fibers mounted in the immp reactor. Ethanol production in isotropic hollow fiber membranebioreactors. In vivo hollow fiber model proqinase drug discovery in. May 26, 2016 this manuscript describes in detail the setup and operation of a hollow fiber bioreactor system for the in vivolike culture of mammalian cells. Figure 1 illustrates the flow pattern and pressure distribution in the hollow fiber. Selection of a compound with the best in vivo activity against a tumor cell line of interest compound screening identification of the most suitable cell line for an in vivo efficacy study with a selected test compound tumor model screening. Hollow fiber reactors have been developed for many biochemical and biomedical applications. Selection of a compound with the best in vivo activity against a tumor cell line of interest compound screening. This thesis focuses on the development of a catalytic hollow fibre membrane reactor for the combined steam methane reforming smr and watergas shift wgs reaction, using a nibased catalyst, and at a temperature window suitable for harvesting pure h2, a clean energy carrier, from.
The hollowfiber membrane systems have been used as bioreactors years ago due to the. Rabies pathobiology and its rna virus agent lyssavirus. The present invention is concerned with a hollow fibre membrane for use in a membrane supported biofilm reactor msbr or the like, the hollow fibre membrane comprising a substantially cylindrical sidewall defining an internal lumen from which gas can permeate through the sidewall, and characterised in that at least a part of an outer surface of the fibre membrane is engineered to define at. Pdf catalytic hollow fibre based reactors for a enhance. Burns doctor of philosophy university of edinburgh. Hydrolysis of butteroil by immobilized lipase using a hollow. A novel hollow fiber membrane biofilm reactor hfmbr was developed to remove nitrate from contaminated drinking water using molecular hydrogen as a clean electrondonor substrate. Jul 09, 2016 however, implementing smallscale hollow fiber bioreactors for culturing mammalian cells can save a lot of time and money, and is especially useful for harvesting large quantities of secreted protein. Doran, in bioprocess engineering principles second edition, 20. A study of polynucleotide phosphorylase production by. This manuscript describes in detail the setup and operation of a hollow fiber bioreactor system for the in vivolike culture of mammalian cells. A 30l hollow fibre reactor with continuous fermentation for cell recycling of escherichia coli as 1. Graphical representation of tissue engineering using hollow fibers as scaffolds.
Hollow fiber membrane bundles are widely used as liquidtoair contactors. Feed enters at one end of the module and retentate leaves at the other end. The performance of hfc reactors is compared with shakeflask reactors using diclofenac as a model substrate. Catalytic hollow fibre membrane reactors for h production. The hff is composed of around one hundred modules each of which contains several thousand hollow fibers soaking in unprocessed water. Alphagalactosidase production and use in a hollowfiber. When enzymes are entrapped in the hollow fiber lumen, substrate mole cule can approach the enzyme directly, thus avoiding the reaction controlled by permeation. As a module, hollow fiber membranes are packed together to form a bundle which is then placed in a shell and sealed at both ends.
Hollow fibre enzymatic reactor, modeling of springerlink. With mhfer, enzymatic digestion of protein could be achieved by continuous flow within a very small volume. These reactors offer a very gentle means for immobilizing. Development of a catalytic hollow fibre membrane micro. Department of chemical engineering and materials science, university of minnesota, minneapolis, mn 55455.
Biological cultures are most commonly performed in flasks, roller bottles or spinners. Wo20155977a1 an aerated biofilm reactor hollow fibre. The hollow fiber reactor hfr is one type of reactor that has the potential for achieving appropriate mass transfer rates for biofuels production, but a better understanding of mass transfer limitations associated with all syngas species is critical for assessing syngas usage during fermentation. In this study, asymmetric al2o3 hollow fibres have been used as a support for either a cuzngaox based catalyst or a pdag membrane in the development of a catalytic hollow fibre micro reactor. This thesis focuses on the development of a catalytic hollow fibre. The potential application of hollow fiber bioreactors to.
Crud is trapped on the outer surface of the hollow fiber and effused water forms an internal flow directed toward the one open end. The hollow fiber bioreactor system delivers media to the cells in a manner akin to the delivery of blood through the capillary networks in vivo. Catalytic hollow fibre membrane reactors for h2 production. Pdf hollow fiber bioreactor technology for tissue engineering. In this study, we report the development of a microbore hollow fiber enzyme reactor mhfer coupled to nanoflow liquid chromatographytandem mass spectrometry nlcesimsms for the online digestion or selective enrichment of glycopeptides and analysis of proteins. Catalytic hollow fibre based reactors for a enhance h2 production by methanol steam reforming. Hollow fiber reactors reduce cost, scaleup concerns by angelo depalma pharmaceutical manufacturers producing up to about 100 kgyr of therapeutic or diagnostic antibodies may soon replace roller bottles, ascites, stirred tanks, and airlift fermenters with hollow fiber bioreactors. A hollow fiber bioreactor is a 3 dimensional cell culturing system based on hollow fibers, which. In the study of these reactor systems, we have used single fiber reactors as a prototype for the larger hollow fiber cartridges.
Harvest large quantities of secreted protein with hollow. View enhanced pdf access article on wiley online library html view download pdf for offline viewing. Hollow fibre reactor a fermentation system in which the cells are separated from the medium using semipermeable membranes arranged in the form of hollow fibres. Hollow fiber bioreactors have been in use for more than 30 years for the production of secreted proteins and antibodies from mammalian cells. Production of exosomes in a hollow fiber bioreactor. This article describes development of a catalytic hollow fibre membrane micro reactor chfmmr for high purity h 2 production. Pdf hollow fibre bioreactor for wastewater treatment using.
Hollow fiber postcolumn reactor for liquid chromatography. Compact hollow fibre reactors for efficient methane conversion article pdf available in journal of the european ceramic society 3716 april 2017 with 20 reads how we measure reads. Amundson, hydrolysis of butteroil by immobilized lipase using a hollow. A novel hollow fiber membrane reactor for high purity h 2. Mass transfer limitations in diffusionlimited isotropic hollow fiber bioreactors. Experiments using single fibers have been conducted to obtain conversion data for reactor scaleup. These hollow fiber membranes are often bundled and housed within tubular polycarbonate shells to create hollow fiber bioreactor cartridges.
A strategy to determine operating parameters in tissue. Microstructured catalytic hollow fibre reactor for methane. Pdf compact hollow fibre reactors for efficient methane. Fibercell systems hollow fibre bioreactor for high density.
Microstructured catalytic hollow fibre reactor for. Present and future in the biotechnology industry jose m. Hollow fibre reactor definition and examples biology. Catalytic hollow fibre membrane reactors for h 2 production ana maria gouveia gil a thesis submitted for the degree of doctor of philosophy and the diploma of imperial college london february 2015. The complete description of the transport of fluid in hollow fiber capillary membrane requires that the local transport equations that describe the flow and transport conditions should be solved.